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81.
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R. B. H. Schutgens G. Schrakamp R. J. A. Wanders H. S. A. Heymans H. W. Moser A. E. Moser J. M. Tagers H. V. D. Bosch P. Aubourm 《黑龙江环境通报》1985,5(5):337-344
Prenatal diagnosis of the cerebro-hepato-renal (Zellweger) syndrome has been performed in 10 pregnancies at risk by measuring both the activity of acyl CoA: dihydroxyacetonephosphate acyltransferase (DHAP-AT) and the de novo plasmalogen biosynthesis, either in cultured amniotic fluid cells or in fibroblasts cultured from a chorionic villus biopsy. In 7 of the pregnancies both tests indicated no abnormality. All 7 continued to term and normal infants were delivered. However, in amniotic fluid cells from 2 fetuses affected by Zellweger syndrome unequivocal differences from control values were found. The activity of DHAP-AT was clearly deficient and the de novo plasmalogen biosynthesis was impaired. In one pregnancy at risk prenatal diagnosis was performed during the first trimester by measuring both the DHAP-AT activity and the de novo plasmalogen biosynthesis in fibroblasts cultured from a chorionic villi biopsy. From the deficient DHAP-AT activity and the impaired de novo plasmalogen biosynthesis it was concluded that the fetus was affected. This was confirmed biochemically after induced abortion. It can be concluded that measurement of the DHAP-AT activity and the de novo plasmalogen biosynthesis provides convenient methods for the early prenatal detection of Zellweger syndrome. 相似文献
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Synthetic hydrophilic polymers find promising applications in pharmacology, biotechnology and chemistry. The biocompatibility, biodegradability and pharmacological activity of these polymers depend much on their hydrophilic nature. This article summarizes the recent developments in the utilization of the different classes of these hydrophilic polymers as pharmacologically active agents, for enzyme modification and as catalysts and supports for chemical reactions. 相似文献
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V. Braun 《Die Naturwissenschaften》1977,64(3):126-132
The resistance of mainly gram-negative bacteria against antibiotics is discussed with respect to new discoveries on the permeability barrier of the outer membrane. Proteins originally characterized as virus receptors have been found to be involved in the translocation of certain substrates across the membrane. Antibiotics can reach their target by the same “pores”. 相似文献
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V. P. Whittaker 《Die Naturwissenschaften》1977,64(12):606-611
The electric organ of Torpedo, besides providing abundant amounts of cholinoceptive post-synaptic membrane for the isolation of the acetylcholine receptor protein, is a rich source of cholinergic nerve terminals. Using perfused, innervated tissue blocks from which synaptic vesicles in different functional states can be isolated, much information can be obtained about synaptic-vesicle dynamics. So far this is consistent with the view that the synaptic vesicles are the source of transmitter released on stimulation and that uptake of newly synthesized transmitter by the vesicles is dependent on their having discharged their previous charge of transmitter in at least one cycle of exo- and endocytosis. Studies of the protein composition of the vesicle membrane, especially when combined with similar information about the external presynaptic membrane, purified samples of which are now available from synaptosome (T-sac) preparations, promise to throw new light on the molecular mechanism underlying vesicle exo-/endocytosis. 相似文献